![]() ![]() These phenotypes were verified by genetic transductants and complemented strains. Three gene mutations not previously associated with cell death: purB, lspA, and clpP were found to be significantly attenuated in their ability to induce neutrophil lysis. aureus mutants, a number of known regulators of cell lysis (including the master regulators accessory gene regulator A, agrA and Staphylococcus exoprotein expression protein S, saeS) were identified in this blinded screen, providing validity to the experimental system. aureus strain, USA300, for decreased ability to induce neutrophil cell lysis. To do this we screened the genome-wide Nebraska Transposon Mutant Library (NTML) in the community acquired methicillin resistant S. aureus-induced neutrophil lysis, and how this contributes to pathogenesis in a whole organism model of infection. ![]() The aim of this study was to better understand the mechanisms underpinning S. aureus, yet this microorganism uses multiple evasion strategies to avoid killing by these immune cells, perhaps the most catastrophic of which is the rapid induction of neutrophil cell death. Neutrophils are essential during the innate immune response to S. As a result of this, there is a great need to better understand how S. Staphylococcus aureus is a commensal organism in approximately 30% of the human population and colonization is a significant risk factor for invasive infection. 3Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom.2Florey Institute, University of Sheffield, Sheffield, United Kingdom.1Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, United Kingdom.Dingyi Yang 1,2, Yin Xin Ho 1,2, Laura M. ![]()
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